2 1 2 cell culture clu209 cells (Cedarlane)
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2 1 2 Cell Culture Clu209 Cells, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2+1+2+cell+culture+clu209+cells/Embryonic+Rat+Hypothalamic+Cell+Line+R18+(rHypoE-18)/pmc02949499-49-0-8
Average 92 stars, based on 3 article reviews
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1) Product Images from "Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge"
Article Title: Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge
Journal:
doi: 10.1016/j.neuroscience.2010.08.015
Figure Legend Snippet: Western blots from CLU209 cells and rat PVN (A) showing the labeling of the neurokinin 3 receptor (NK3R, sheep anti-NK3R in CLU209, K7 anti-NK3R in rat) and importin β-1 (Imp β-1, KPNB1 labeling for both samples). RT-PCR from the CLU209 and rat PVN (B) also demonstrate that mRNA for NK3R and imp β-1 are expressed in these samples along with vasopressin (VP). The center marker shows the 300 bp, 150 bp and 50 bp bands respectively.
Techniques Used: Western Blot, Labeling, Reverse Transcription Polymerase Chain Reaction, Marker
Figure Legend Snippet: Quantification of the labeling density of nuclear neurokinin 3 receptor (NK3R) in the CLU209 cells following importin β-1 immuno-neutralization. Lamin B was used as an internal loading control. The decrease in the nuclear presence of the NK3R was dependent on the time of incubation with the importin β-1 antibody.
Techniques Used: Labeling, Neutralization, Control, Incubation
Figure Legend Snippet: Western blots from CLU209 cell homogenate following incubation with the anti importin β-1 antibody. These data show that there was no decrease in the expression levels of neurokinin 3 receptor (NK3R, sheep anti-NK3R antibody) or lamin B following incubation with the importin β-1 antibody. These data also show that there was no activation of caspases-3 or increase in cell death due to incubation with the antibody.
Techniques Used: Western Blot, Incubation, Expressing, Activation Assay
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